What is the composition of peptone?
The two basic components of peptone water are peptone and sodium chloride. Peptone provides nitrogenous and carbonaceous compounds, long-chain amino acids, vitamins provide essential nutrients. Sodium chloride provides the necessary electrolyte and maintains the osmotic balance of the medium.
What is the composition of glucose in glucose broth?
Ingredients
| Ingredients | g/L |
|---|---|
| Tryptone | 10.0 |
| Glucose | 5.0 |
| Sodium Chloride | 5.0 |
Which type of culture media is peptone water?
Peptone Water is a type of broth medium. This media is mainly used to grow the organism and is also used as a base for determining carbohydrate fermentation patterns of non-fastidious organisms.
How peptone is made?
Peptone, a protein decomposition product, is made by incomplete hydrolysis process of the protein originated from beef, casein, milk powder, gelatin, soy protein, silk protein, fibrin, etc. The commercially available products are mainly light yellow to brown yellow powder.
What nutrients do peptone contain?
Peptones are water-soluble protein hydrolysates, containing peptides, amino acids, and inorganic salts as well as other compounds, such as lipids, vitamins, and sugars [5].
How do you make Peptone Water?
Preparation Instructions Suspend 15 grams of Peptone Water in 1000 ml of distilled water. Add the test carbohydrate in the desired quantity and dissolve completely. Dispense in tubes with or without inverted Durham’s tubes and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 minutes.
How do you make glucose phosphate broth?
Preparation of glucose phosphate peptone water:
- Dissolve the peptone and phosphate salt in the water by steaming.
- Add the glucose and mix well.
- Dispense the medium in 2 ml amounts in small screw-cap tubes or bottles.
- Sterilize by autoclaving (with cap loosened) at 115°C for 10 minutes.
How do you make alkaline peptone water?
Preparation of Alkaline Peptone Water
- To make about 50 bottles (i.e. 500 ml), you will need 5 g of peptone and 5 g of Sodium chloride.
- Dissolve the peptone and sodium chloride in the water.
- Adjust the reaction of the medium to pH 8.6 to 9.0 using 1 mol/L sodium hydroxide.
Why Peptone Water is used in microbiology?
Peptone Water is used as a growth medium and as the basis of carbohydrate fermentation media. Peptone Water is recommended for studying the ability of an organism to ferment a specific carbohydrate, which aids in the differentiation of genera and species.
Which type of media contain basic nutrients like peptone mineral salt and water?
Simple media: It is also called basal media. It consists of meat extract, peptone, sodium chloride and water.
How do you make peptone water?
How do you make buffered peptone water?
Suspend 20.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in 50 ml amounts into tubes or flasks or as desired. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.
What are the two components of peptone water?
Principle The two basic components of peptone water are peptone and sodium chloride. Peptone provides nitrogenous and carbonaceous compounds, long-chain amino acids, vitamins provide essential nutrients. Sodium chloride provides the necessary electrolyte and maintains the osmotic balance of the medium.
What are the ingredients in the peptone test?
Ingredients Gms / Litre Peptone 10.000 Sodium chloride 5.000 Final pH ( at 25°C) 7.2±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 15.0 grams in 1000 ml distilled water. Add the test carbohydrate in desired quantity and dissolve completely.
What is the final pH of peptone water?
Final pH (at 25°C) 7.2±0.2 Suspend 15.0 grams in 1000 ml distilled water. Mix thoroughly and distribute into the final containers. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. The two basic components of peptone water are peptone and sodium chloride.
What are the ingredients in Pepto-Bismol?
Ingredients Gms / Litre Buffered peptone 7.000 Dextrose (Glucose) 5.000 Dipotassium phosphate 5.000 Final pH ( at 25°C) 6.9±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 17.0 grams in 1000 ml of purified / distilled water.