What is NLuc?

NanoLuc™ (Nluc) is a 19.1 kDa, ATP- independent luciferase that utilizes a novel coelenterazine analog (furimazine) to produce high intensity, glow-type luminescence.

How does Gaussia luciferase work?

Gaussia luciferase can be expressed in mammalian cells using commercially available reporter plasmids. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate coelenterazine in a reaction that emits light (at 470 nm), and has considerable advantages over other reporter genes.

Is luciferase monomeric or dimeric?

Firefly luciferase is extremely hydrophobic; the monomer is apparently active, but the enzyme exists as a dimer in most solutions [6].

Is luciferase a dimeric protein?