What is mCherry fluorescence?
mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity.
What is a mCherry reporter?
Afp::mCherry, a red fluorescent transgenic reporter of the mouse visceral endoderm. Genesis.
What Colour is mCherry?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed.
Is mCherry a reporter gene?
Our results identified mCherry as a reliable and quantitative reporter for the study of gene expression in chloroplasts, which is essential for the biotechnological application of microalgal chloroplasts and for improved production of valuable recombinant proteins.
What laser excites mCherry?
561-nm
The 561-nm (yellow-green) laser results in more efficient excitation (64%) of mCherry because this wavelength is closer to the maximum excitation wavelength than the 488-nm (blue) laser. The 561-nm laser therefore results in a brighter emission signal as shown in Figure 6.
Why is mCherry a good model system?
mCherry, out of all of the true monomers developed, has the longest wavelengths, the highest photostability, fastest maturation, excellent pH resistance, and is closest to mRFP1 in its excitation and emission maxima.
Where is mCherry from?
sea anemones
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments.
Is mCherry far-red?
mCherry is an excellent fluorescent protein that has maximal emittance in the red spectral range and a high fluorescence quantum yield, and thus, can be used as a donor for energy transfer to a far-red acceptor, such as BDFP1. 6, by FRET. 6 efficiently; thus emitting strong far-red fluorescence maximally at 666 nm.