How do you dilute SYBR gold?
Dilute the Sybr® Gold reagent 1:100 in 1X TE pH 8.0 (The 1:100 dilution may be stable for up to 24 hours if refrigerated and protected from light.) *To make a 1:100 dye dilution transfer 10 ul of 10,000X stock Sybr® Gold Dye into 990 ul 1x TE into a clean amber or foil covered 1.5 mL microfuge tube.
Can you microwave SYBR Safe?
SYBR Safe DNA Gel Stain may be briefly microwaved with no loss of performance.
Can you reuse SYBR Gold?
SYBR® Gold Nucleic Acid Gel Stain | 4 The staining solution may be stored in the dark and can be reused 3–4 times, although best results are obtained from fresh staining solution.
Is SYBR Gold toxic?
Mutagenicity of these stains was not observed although their toxic concentration was reached. Toxic effects of SYBR Green II and SYBR Gold were seen approximately at the same molar concentrations as reported previously for SYBR Green I.
How do you make SYBR Safe DNA gel stain?
METHOD II: POST-ELECTROPHORESIS SYBR® SAFE DNA STAINING PROTOCOL
- DILUTE SYBR® Safe 1: 10,000 by adding 7.5 μl of the concentrated stain to 75 ml of 1x electrophoresis buffer in a flask.
- REMOVE the agarose gel and casting tray from the electrophoresis chamber.
- POUR the 1x SYBR® Safe stain solution over the gel.
How do you stain SYBR Gold with gel?
- 1.1 Dilute the stock SYBR® Gold stain 10,000-fold to make a 1X staining solution.
- 1.2 Incubate the gel in 1X staining solution for 10–40 minutes.
- 1.3 Agitate the gel gently at room temperature.
- 2.1 Illuminate the stained gel.
- 2.2 Photograph the gel.
How does SYBR Safe stain DNA?
SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
Can you add SYBR Safe to hot agarose?
Can agarose gels be cast with SYBR Safe DNA Gel Stain in them? Yes. Simply substitute a SYBR Safe DNA Gel Stain solution for the buffer when preparing the molten agarose.
What is the characterization of SYBR gold gel stain?
Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators. Anal Biochem (1999) 268:278-288. (PMID: 10075818) For Research Use Only. Not for use in diagnostic procedures. Sensitivity of SYBR® Gold stain compared to silver stain.
What is the difference between SYBR Green I and SYBR gold?
SYBR Gold was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.
What is asysybr gold stain?
SYBR® Gold stain is a proprietary unsymmetrical cyanine dye that exhibits >1000-fold fluorescence enhancement upon binding to nucleic acids and has a high quantum yield (∼0.6) upon binding to double- or single-stranded DNA or to RNA 1.
What is the sensitivity of SYBR Green I?
SYBR® Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR® Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results.